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This article is part of the supplement: 21st International Symposium on Intensive Care and Emergency Medicine

Open Badges Meeting abstract

Accuracy of blood volume measurement using an integrated fiberoptic monitoring system in septic shock

G Marx1, M Cobas Meyer2, T Schuerholz2, B Vangerow2, T Simon2, KF Gratz3 and M Leuwer1

Author Affiliations

1 University Department of Anaesthesia, University of Liverpool, L69 3GA, UK

2 Department of Anaesthesia

3 Department of Nuclear Medicine, Hannover Medical School, Germany

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Critical Care 2001, 5(Suppl 1):P159-S74  doi:10.1186/cc1226

The electronic version of this article is the complete one and can be found online at:

Received:15 January 2001
Published:2 March 2001



To compare the accuracy of an integrated fiberoptic monitoring system in measuring blood volume (BV) with standard method using chromium-51-tagged erythrocytes in septic shock.


Prospective animal laboratory study.

Measurement and main results

Twenty anaesthetised, and mechanically ventilated pigs (20.9 ± 1.9 kg) were investigated over a period of 8 h. Septic shock was induced with faecal peritonitis (1 g/kg body weight autologous faeces). A central venous catheter was used for the injection of the indicator dyes. BV was measured by detecting indocyanin green by reflection densitrometry using a fiberoptic thermistor tipped catheter inserted into right carotid artery (4F PV 2024, Pulsion Medical Systems). Haemodynamic treatment scheme was aimed at maintenance of a central venous pressure of 12 mmHg. Data were analysed using Bland-Altman analyses, linear regression and correlation. Forty data pairs of simultaneous BV-measurements were yielded during haemodynamic consistency with a mean BV measured by integrated fiberoptic monitoring system of 66.6 ± 20.3 ml/kg (range: 24.5–122.6 ml/kg). Mean BV measured by chromium-51-tagged erythrocytes was 76.1 ± 17.9 ml/kg (range: 49.7–121.6 ml/kg). Linear regression equation was: BV by integrated fiberoptic monitoring system = 0.65 × BV: chromium-51-tagged erythrocytes + 17.6; r = 0.57, P < 0.01. The mean bias was 9.6 ml/kg (95% confidence interval: 3.7–15.4 ml/kg), with limits of agreement of –26.5 to 45.6 ml/kg and a precision of 16.8 ml/kg.


In this model of porcine septic shock we could show a significant correlation in blood volume measurement between fiberoptic monitoring system and chromium-51-tagged erythrocytes. The relatively wide limits of agreement might be due to pronounced circulatory alterations including slow mixing compartments, prolonged equilibration and sequestration in septic shock.