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Whole blood impedance aggregometry findings in experimental endotoxinemia

Vivian Simm, Daniel Dirkmann, Jürgen Peters and Matthias Hartmann*

Author Affiliations

Klinik für Anästhesiologie und Intensivmedizin, Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany

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Critical Care 2013, 17:427  doi:10.1186/cc12583

See related research by Adamzik et al.,

Published: 17 April 2013

First paragraph (this article has no abstract)

In an observational study in a recent issue of Critical Care, we demonstrated that platelet function, as assessed by impedance aggregometry, is reduced in patients with severe sepsis and poor prognosis [1]. In the present study, we investigated whether the prototypic endotoxin lipopolysaccharide (LPS) (Escherichia coli; serotype 0.111:B4; Sigma-Aldrich, Munich, Germany) exerts comparable effects in an in vitro model. Therefore, heparinized blood samples were drawn from the antecubital vein of healthy probands. After incubation of samples with LPSs (100 μg/mL) or vehicle for 240 minutes at 37°C, samples were subjected to impedance aggregometry (Roche Diagnostics Deutschland GmbH, Mannheim, Germany), and aggregation was induced by adenosine diphosphate (ADP), collagen, thrombin receptor-activating peptide (TRAP), or arachidonic acid.